Impact of Ammonium Permeases MepA, MepB, and MepC on Nitrogen-Regulated Secondary Metabolism in Fusarium fujikuroi

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Impact of ammonium permeases mepA, mepB, and mepC on nitrogen-regulated secondary metabolism in Fusarium fujikuroi.

In Fusarium fujikuroi, the production of gibberellins and bikaverin is repressed by nitrogen sources such as glutamine or ammonium. Sensing and uptake of ammonium by specific permeases play key roles in nitrogen metabolism. Here, we describe the cloning of three ammonium permease genes, mepA, mepB, and mepC, and their participation in ammonium uptake and signal transduction in F. fujikuroi. The...

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The impact of the ammonium permeases MepA , MepB 1 and MepC on nitrogen - regulated secondary 2 metabolism in Fusarium fujikuroi 3 4

4 Sabine Teichert 1,2 , Julian C. Rutherford 3,4 , Marieke Wottawa 1,2 , Joseph Heitman 3 , and 5 Bettina Tudzynski 1* 6 7 1 Institut für Botanik der Westfälischen Wilhelms-Universität Münster, Schloßgarten 3, 8 D-48149 Münster, Germany 9 2 Current address, Institut für Physiologie, Abteilung Herzund Kreislaufphysiologie, Georg10 August-Universität Göttingen, Humboldtallee 23, D-37073 Göttingen...

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Role of the Fusarium fujikuroi TOR kinase in nitrogen regulation and secondary metabolism.

In Fusarium fujikuroi, the biosynthesis of gibberellins (GAs) and bikaverin is under control of AreA-mediated nitrogen metabolite repression. Thus far, the signaling components acting upstream of AreA and regulating its nuclear translocation are unknown. In Saccharomyces cerevisiae, the target of rapamycin (TOR) proteins, Tor1p and Tor2p, are key players of nutrient-mediated signal transduction...

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The General Amino Acid Permease FfGap1 of Fusarium fujikuroi Is Sorted to the Vacuole in a Nitrogen-Dependent, but Npr1 Kinase-Independent Manner

The rice pathogenic fungus Fusarium fujikuroi is well known for the production of a broad spectrum of secondary metabolites (SMs) such as gibberellic acids (GAs), mycotoxins and pigments. The biosynthesis of most of these SMs strictly depends on nitrogen availability and of the activity of permeases of nitrogen sources, e.g. the ammonium and amino acid permeases. One of the three ammonium perme...

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Comparative transcriptome and proteome analysis reveals a global impact of the nitrogen regulators AreA and AreB on secondary metabolism in Fusarium fujikuroi

The biosynthesis of multiple secondary metabolites in the phytopathogenic ascomycete Fusarium fujikuroi is strongly affected by nitrogen availability. Here, we present the first genome-wide transcriptome and proteome analysis that compared the wild type and deletion mutants of the two major nitrogen regulators AreA and AreB. We show that AreB acts not simply as an antagonist of AreA counteracti...

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ژورنال

عنوان ژورنال: Eukaryotic Cell

سال: 2008

ISSN: 1535-9778,1535-9786

DOI: 10.1128/ec.00351-07